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Flow cytometry analysis of targeted contrast agent-labeled cancer cells and <t>peripheral</t> blood <t>mononuclear</t> cells <t>(PBMCs).</t> Suspensions of 1:1000 IGROV-1 ovarian cancer cells to PBMCs were incubated (a) without and (b) with OTL38. (c) Different FR expressing cancer cell lines and PBMCs incubated with OTL38 compared with unlabeled PBMCs, cells, and reference μspheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color. Suspensions of 1:1000 LLC lung cancer to PBMCs were incubated (d) without and (e) with VGT-309. (f) Different cathepsin-expressing cancer cell lines and PBMCs incubated with VGT-309 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color.
Peripheral Blood Mononuclear Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbmcs  (iXCells Biotechnologies)
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Flow cytometry analysis of targeted contrast agent-labeled cancer cells and <t>peripheral</t> blood <t>mononuclear</t> cells <t>(PBMCs).</t> Suspensions of 1:1000 IGROV-1 ovarian cancer cells to PBMCs were incubated (a) without and (b) with OTL38. (c) Different FR expressing cancer cell lines and PBMCs incubated with OTL38 compared with unlabeled PBMCs, cells, and reference μspheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color. Suspensions of 1:1000 LLC lung cancer to PBMCs were incubated (d) without and (e) with VGT-309. (f) Different cathepsin-expressing cancer cell lines and PBMCs incubated with VGT-309 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color.
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pbmc  (Miltenyi Biotec)
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Flow cytometry analysis of targeted contrast agent-labeled cancer cells and <t>peripheral</t> blood <t>mononuclear</t> cells <t>(PBMCs).</t> Suspensions of 1:1000 IGROV-1 ovarian cancer cells to PBMCs were incubated (a) without and (b) with OTL38. (c) Different FR expressing cancer cell lines and PBMCs incubated with OTL38 compared with unlabeled PBMCs, cells, and reference μspheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color. Suspensions of 1:1000 LLC lung cancer to PBMCs were incubated (d) without and (e) with VGT-309. (f) Different cathepsin-expressing cancer cell lines and PBMCs incubated with VGT-309 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color.
Pbmc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbmc/product/Miltenyi Biotec
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biological samples human peripheral blood mononuclear cells pbmcs  (ATCC)
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Flow cytometry analysis of targeted contrast agent-labeled cancer cells and <t>peripheral</t> blood <t>mononuclear</t> cells <t>(PBMCs).</t> Suspensions of 1:1000 IGROV-1 ovarian cancer cells to PBMCs were incubated (a) without and (b) with OTL38. (c) Different FR expressing cancer cell lines and PBMCs incubated with OTL38 compared with unlabeled PBMCs, cells, and reference μspheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color. Suspensions of 1:1000 LLC lung cancer to PBMCs were incubated (d) without and (e) with VGT-309. (f) Different cathepsin-expressing cancer cell lines and PBMCs incubated with VGT-309 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color.
Biological Samples Human Peripheral Blood Mononuclear Cells Pbmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biological samples human peripheral blood mononuclear cells pbmcs/product/ATCC
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Flow cytometry analysis of targeted contrast agent-labeled cancer cells and <t>peripheral</t> blood <t>mononuclear</t> cells <t>(PBMCs).</t> Suspensions of 1:1000 IGROV-1 ovarian cancer cells to PBMCs were incubated (a) without and (b) with OTL38. (c) Different FR expressing cancer cell lines and PBMCs incubated with OTL38 compared with unlabeled PBMCs, cells, and reference μspheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color. Suspensions of 1:1000 LLC lung cancer to PBMCs were incubated (d) without and (e) with VGT-309. (f) Different cathepsin-expressing cancer cell lines and PBMCs incubated with VGT-309 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color.
Cd3 T Cell Pbmc Mixtures, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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irradiated pbmc  (iQ Biosciences)
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iQ Biosciences irradiated pbmc
Flow cytometry analysis of targeted contrast agent-labeled cancer cells and <t>peripheral</t> blood <t>mononuclear</t> cells <t>(PBMCs).</t> Suspensions of 1:1000 IGROV-1 ovarian cancer cells to PBMCs were incubated (a) without and (b) with OTL38. (c) Different FR expressing cancer cell lines and PBMCs incubated with OTL38 compared with unlabeled PBMCs, cells, and reference μspheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color. Suspensions of 1:1000 LLC lung cancer to PBMCs were incubated (d) without and (e) with VGT-309. (f) Different cathepsin-expressing cancer cell lines and PBMCs incubated with VGT-309 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color.
Irradiated Pbmc, supplied by iQ Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cd4 t cell purification human pbmcs  (Miltenyi Biotec)
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Miltenyi Biotec human cd4 t cell purification human pbmcs
Flow cytometry analysis of targeted contrast agent-labeled cancer cells and <t>peripheral</t> blood <t>mononuclear</t> cells <t>(PBMCs).</t> Suspensions of 1:1000 IGROV-1 ovarian cancer cells to PBMCs were incubated (a) without and (b) with OTL38. (c) Different FR expressing cancer cell lines and PBMCs incubated with OTL38 compared with unlabeled PBMCs, cells, and reference μspheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color. Suspensions of 1:1000 LLC lung cancer to PBMCs were incubated (d) without and (e) with VGT-309. (f) Different cathepsin-expressing cancer cell lines and PBMCs incubated with VGT-309 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color.
Human Cd4 T Cell Purification Human Pbmcs, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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blood mononuclear cells  (ATCC)
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ATCC blood mononuclear cells
Flow cytometry analysis of targeted contrast agent-labeled cancer cells and <t>peripheral</t> blood <t>mononuclear</t> cells <t>(PBMCs).</t> Suspensions of 1:1000 IGROV-1 ovarian cancer cells to PBMCs were incubated (a) without and (b) with OTL38. (c) Different FR expressing cancer cell lines and PBMCs incubated with OTL38 compared with unlabeled PBMCs, cells, and reference μspheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color. Suspensions of 1:1000 LLC lung cancer to PBMCs were incubated (d) without and (e) with VGT-309. (f) Different cathepsin-expressing cancer cell lines and PBMCs incubated with VGT-309 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color.
Blood Mononuclear Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Flow cytometry analysis of targeted contrast agent-labeled cancer cells and peripheral blood mononuclear cells (PBMCs). Suspensions of 1:1000 IGROV-1 ovarian cancer cells to PBMCs were incubated (a) without and (b) with OTL38. (c) Different FR expressing cancer cell lines and PBMCs incubated with OTL38 compared with unlabeled PBMCs, cells, and reference μspheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color. Suspensions of 1:1000 LLC lung cancer to PBMCs were incubated (d) without and (e) with VGT-309. (f) Different cathepsin-expressing cancer cell lines and PBMCs incubated with VGT-309 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color.

Journal: Journal of Biomedical Optics

Article Title: Considerations for the use of targeted fluorescence contrast agents to detect circulating cancer cell populations with diffuse in vivo flow cytometry

doi: 10.1117/1.JBO.31.2.027001

Figure Lengend Snippet: Flow cytometry analysis of targeted contrast agent-labeled cancer cells and peripheral blood mononuclear cells (PBMCs). Suspensions of 1:1000 IGROV-1 ovarian cancer cells to PBMCs were incubated (a) without and (b) with OTL38. (c) Different FR expressing cancer cell lines and PBMCs incubated with OTL38 compared with unlabeled PBMCs, cells, and reference μspheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color. Suspensions of 1:1000 LLC lung cancer to PBMCs were incubated (d) without and (e) with VGT-309. (f) Different cathepsin-expressing cancer cell lines and PBMCs incubated with VGT-309 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and NIR-DiFC detection threshold (red line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color.

Article Snippet: Peripheral blood mononuclear cells (PBMCs; PCS-800-011; ATCC) were thawed and prepared according to ATCC instructions for in vitro non-specific contrast agent uptake studies.

Techniques: Flow Cytometry, Labeling, Incubation, Expressing

Flow cytometry analysis of targeted contrast agent-labeled cancer cells and PBMCs. Suspensions of 1:1000 LNCaP prostate cancer cells to PBMCs were incubated (a) without and (b) with PSMA-02. (c) Different PSMA-expressing cancer cell lines and PBMCs incubated with PSMA-02 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and Red-NIR-DiFC detection threshold (green line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color. Suspensions of 1:1000 LNCaP prostate cancer to PBMCs were incubated (d) without and (e) with PSMA-04. (f) Different PSMA-expressing cancer cell lines and PBMCs incubated with PSMA-04 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and Red-NIR-DiFC detection threshold (green line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color.

Journal: Journal of Biomedical Optics

Article Title: Considerations for the use of targeted fluorescence contrast agents to detect circulating cancer cell populations with diffuse in vivo flow cytometry

doi: 10.1117/1.JBO.31.2.027001

Figure Lengend Snippet: Flow cytometry analysis of targeted contrast agent-labeled cancer cells and PBMCs. Suspensions of 1:1000 LNCaP prostate cancer cells to PBMCs were incubated (a) without and (b) with PSMA-02. (c) Different PSMA-expressing cancer cell lines and PBMCs incubated with PSMA-02 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and Red-NIR-DiFC detection threshold (green line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color. Suspensions of 1:1000 LNCaP prostate cancer to PBMCs were incubated (d) without and (e) with PSMA-04. (f) Different PSMA-expressing cancer cell lines and PBMCs incubated with PSMA-04 compared with unlabeled PBMCs, cells, and reference μ spheres (JGLI). Labeled cell threshold (black line) and Red-NIR-DiFC detection threshold (green line) are shown, and percentages of cell populations above the respective threshold are indicated in the same color.

Article Snippet: Peripheral blood mononuclear cells (PBMCs; PCS-800-011; ATCC) were thawed and prepared according to ATCC instructions for in vitro non-specific contrast agent uptake studies.

Techniques: Flow Cytometry, Labeling, Incubation, Expressing